FIGURE

Fig. 6

ID

ZDB-FIG-171011-23

Publication

Nakayama et al., 2017 - Comprehensive analysis of target genes in zebrafish embryos reveals gbx2 involvement in neurogenesis

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Fig. 6

gbx2 promotes neurogenesis in the midbrain-hindbrain region. Embryos obtained by mating Tg(hsp70l:gbx2) and wild-type fish were subjected to heat shock at the bud stage and analyzed at 24 hpf for the expression of neurogenesis genes by WISH. The genotypes of the stained embryos were confirmed by PCR (a–j, sibling; a'–j', hsp-gbx2+). Dorsal views with anterior to the left (a, c, e, g, i, a', c', e', g', i') and lateral views with anterior to the left and dorsal to the top (b, d, f, h, j, b', d', f', h', j') are shown. Arrows, ectopic expression; cer, cerebellum; basal r1, basal region of rhombomere 1; cteg, caudal tegmentum; di, diencephalon; gngl, cranial ganglion; ht, hypothalamus; ptec, pretectum; pth, prethalamus; r1/r3/r5, rhombomere 1/3/5; te, telencephalon; tec, tectum; teg, tegmentum; tg, trigeminal ganglion; black arrowheads, normal MHB; open arrowheads, abnormal MHB. Scale bars, 200 µm.

Expression Data

Genes:	atoh1a elavl3 epha4a neurog1 rorab
Fish:	WT sud12Tg/+
Condition:	heat shock
Anatomical Terms:	cerebellum diencephalon dorsal region forebrain midbrain neural tube optic tectum optic tectum ventricular zone rhombomere 1 rhombomere 3 rhombomere 5
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sud12Tg/+
Condition:	heat shock
Observed In:	cerebellum diencephalon dorsal region forebrain midbrain optic tectum optic tectum ventricular zone rhombomere 1
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 430(1), Nakayama, Y., Inomata, C., Yuikawa, T., Tsuda, S., Yamasu, K., Comprehensive analysis of target genes in zebrafish embryos reveals gbx2 involvement in neurogenesis, 237-248, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.