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ZFIN ID: ZDB-FIG-171011-16
Morrow et al., 2017 - tbx6l and tbx16 are redundantly required for posterior paraxial mesoderm formation during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   246(10):759-769 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5

A single neural tube forms in tbx6l; tbx16 double mutants. A–F: In situ hybridization for isl1 (A–C) and sox2 (D–F) at 24 hpf. G–L: Confocal projections of embryos labeled for acetylated tubulin (green), fast muscle (F310; red), and myosin heavy chain (A4.1025; blue) in lateral (G–I) and dorsal (J–L) views. Not shown are tbx6l single-mutant embryos as they are indistinguishable from wild-type embryos. Scale bars in A (for A–F) and G (for G–I) = 100 μm. Scale bar in J (for J–L) = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl1 WT standard conditions Prim-5 spinal cord neuron ISH
tbx16b104/b104 standard conditions Prim-5 spinal cord motor neuron ISH
Prim-5 tail bud ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 spinal cord motor neuron ISH
Prim-5 tail bud ISH
sox2 WT standard conditions Prim-5 neuron ISH
tbx16b104/b104 standard conditions Prim-5 hypochord ISH
Prim-5 intermediate mesoderm ISH
Prim-5 neuron ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 hypochord ISH
Prim-5 intermediate mesoderm ISH
Prim-5 neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions Prim-5 hypochord sox2 expression spatial pattern, abnormal
Prim-5 intermediate mesoderm sox2 expression spatial pattern, abnormal
Prim-5 spinal cord motor neuron isl1 expression increased amount, abnormal
Prim-5 tail bud isl1 expression increased distribution, abnormal
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 hypochord sox2 expression spatial pattern, abnormal
Prim-5 intermediate mesoderm sox2 expression spatial pattern, abnormal
Prim-5 spinal cord motor neuron isl1 expression increased amount, abnormal
Prim-5 tail bud isl1 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.