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ZFIN ID: ZDB-FIG-171011-15
Morrow et al., 2017 - tbx6l and tbx16 are redundantly required for posterior paraxial mesoderm formation during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   246(10):759-769 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 4

tbx6l and tbx16 have partially redundant roles in formation of tail paraxial mesoderm. A–R: In situ hybridization for ta (A–C), tbx6 (fss) (D–F), myoD (G–I), pax2a (J–L), fli1a (M–O), and lmo2 (P–R) at 24 hpf (A–C;G–R) and the 14- to 16-somite stage (D–F). Not shown are tbx6l single-mutant embryos as they are indistinguishable from wild-type embryos. In panel J, the row of pax2a-expressing pronephros cells is indicated by an asterisk (*), and the row of pax2a-expressing spinal cord neurons is indicated by a double asterisk (**). Scale bars in C (for A–C) and R (for G–R) = 100 μm. Scale bar in F (for D–F) = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fli1a WT standard conditions Prim-5 vasculature ISH
tbx16b104/b104 standard conditions Prim-5 vasculature ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 vasculature ISH
lmo2 WT standard conditions Prim-5 angioblastic mesenchymal cell ISH
tbx16b104/b104 standard conditions Prim-5 angioblastic mesenchymal cell ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 angioblastic mesenchymal cell ISH
myod1 WT standard conditions Prim-5 myotome ISH
tbx16b104/b104 standard conditions Prim-5 myotome ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 myotome ISH
pax2a WT standard conditions Prim-5 pronephros ISH
Prim-5 spinal cord neuron ISH
tbx16b104/b104 standard conditions Prim-5 pronephros ISH
Prim-5 spinal cord neuron ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions Prim-5 pronephros ISH
Prim-5 spinal cord neuron ISH
tbx6 WT standard conditions 14-19 somites paraxial mesoderm ISH
tbx16b104/b104 standard conditions 14-19 somites paraxial mesoderm ISH
tbx16lz35/z35; tbx16b104/b104 standard conditions 14-19 somites paraxial mesoderm ISH
tbxta WT standard conditions Prim-5 notochord cell ISH
Prim-5 tail bud mesodermal cell ISH
tbx16lz34/z34 standard conditions Prim-5 tail bud mesodermal cell ISH
tbx6te314a/te314a; tbx16lz34/z34 standard conditions Prim-5 tail bud mesodermal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbx16b104/b104 standard conditions Prim-5 angioblastic mesenchymal cell lmo2 expression spatial pattern, abnormal
Prim-5 pronephros pax2a expression increased amount, abnormal
Prim-5 spinal cord neuron disorganized, abnormal
Prim-5 spinal cord neuron pax2a expression increased amount, abnormal
Prim-5 vasculature fli1a expression spatial pattern, abnormal
tbx16lz34/z34 standard conditions Prim-5 tail bud mesodermal cell tbxta expression increased distribution, abnormal
tbx16lz35/z35; tbx16b104/b104 standard conditions 14-19 somites paraxial mesoderm tbx6 expression decreased amount, abnormal
Prim-5 angioblastic mesenchymal cell lmo2 expression spatial pattern, abnormal
Prim-5 myotome myod1 expression decreased amount, abnormal
Prim-5 pronephros pax2a expression increased amount, abnormal
Prim-5 spinal cord neuron disorganized, abnormal
Prim-5 spinal cord neuron pax2a expression increased amount, abnormal
Prim-5 vasculature fli1a expression spatial pattern, abnormal
tbx6te314a/te314a; tbx16lz34/z34 standard conditions Prim-5 tail bud mesodermal cell tbxta expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.