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ZFIN ID: ZDB-FIG-171009-28
Soman et al., 2017 - Inhibition of the mitochondrial calcium uniporter rescues dopaminergic neurons in pink1-/- zebrafish.. The European journal of neuroscience   45(4):528-535 Full text @ Eur. J. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2

Knockdown of mcu leads to rescue of dopaminergic neurons. (A) Dopaminergic (DA) neuronal cell count in wt, wt MCU (wt microinjected with MO against mcu), pink1−/−, pink1−/− MCU (pink1−/− microinjected with MO against mcu) zebrafish larvae at 3 dpf. DA neuronal cell count is reduced in pink1/ (*P = 0.012) but completely rescued after MCU inactivation (**P = 0.0085). The scale on the y axis reflects % of DA neurons compared to wt controls. (B) Representative image showing dopaminergic neurons in the diencephalon [using tyrosine hydroxylase (TH) whole mount in situ (WISH) staining] in wt controls and pink1−/− zebrafish injected with and without mcu MO. (C) Dopaminergic (DA) neuronal cell count in wt, wt embryos treated with Ruthenium red (RR) (wt RR), pink1−/− and pink1−/− embryos treated with RR (pink1−/− RR) with complete rescue of DA neurons in pink1−/− larvae after RR treatment (****P < 0.0001). The scale on the y axis reflects % of DA neurons compared to wt controls. (D) WT vs pink1−/− (***P = 0.0007). Mitochondrial complex I activity is restored in 5 dpf pink1−/− following mcu k/d (**P = 0.0071). Complex I activity is measured in μmol oxidized NADH per 1 unit of citrate synthase activity and set to 100% for the complex I activity in WT controls. The scale on the y axis reflects % of complex I activity compared to wt controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
th WT standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
WT + MO3-mcu standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
pink1sh397/sh397 standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
pink1sh397/sh397 + MO3-mcu standard conditions Protruding-mouth diencephalon dopaminergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: ruthenium red Protruding-mouth diencephalon has number of diencephalon dopaminergic neuron, normal
WT + MO3-mcu standard conditions Protruding-mouth diencephalon has number of diencephalon dopaminergic neuron, normal
Day 5 whole organism NADH dehydrogenase (ubiquinone) activity occurrence, normal
pink1sh397/sh397 standard conditions Protruding-mouth diencephalon has fewer parts of type diencephalon dopaminergic neuron, abnormal
Day 5 whole organism NADH dehydrogenase (ubiquinone) activity decreased occurrence, abnormal
pink1sh397/sh397 chemical treatment by environment: ruthenium red Protruding-mouth diencephalon has number of diencephalon dopaminergic neuron, ameliorated
pink1sh397/sh397 + MO3-mcu standard conditions Protruding-mouth diencephalon has number of diencephalon dopaminergic neuron, ameliorated
Day 5 whole organism NADH dehydrogenase (ubiquinone) activity occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal The European journal of neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Eur. J. Neurosci.