header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171009-27
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.
Soman et al., 2017 - Inhibition of the mitochondrial calcium uniporter rescues dopaminergic neurons in pink1-/- zebrafish.. The European journal of neuroscience   45(4):528-535 Full text @ Eur. J. Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 4-cell to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 1

mcu expression. (A) RT-PCR analysis showing consistent expression of mcu throughout development (1, 24, 48 and 72 hpf). (B) In situ hybridization with dorsal (left) and lateral (right) views of mcu expression in 3 dpf zebrafish larvae, demonstrating strong expression in the brain. (C) Quantification of mcu mRNA at 3 dpf, demonstrating similar expression levels in wt and pink1−/−. (D) RT-PCR analysis of mcu knockdown using antisense morpholino (MO) at 3 dpf, demonstrating the marked effect of MO mediated mcu k/d on mcu wild type transcript levels. (E) Q-PCR based confirmation of marked effect of MO-mediated mcu k/d on mcu mRNA transcript levels (****P < 0.0001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mcu WT standard conditions 4-cell whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth brain ISH
Protruding-mouth whole organism RTPCR
WT + MO3-mcu standard conditions Protruding-mouth whole organism RTPCR
pink1sh397/sh397 standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-mcu standard conditions Protruding-mouth whole organism mcu expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The European journal of neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Eur. J. Neurosci.