| ZFIN ID: ZDB-FIG-171009-27 |
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Terms: | |
| Stage Range: | 4-cell to Protruding-mouth |
| Fish: | |
|---|---|
| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Protruding-mouth |
Fig. 1 mcu expression. (A) RT-PCR analysis showing consistent expression of mcu throughout development (1, 24, 48 and 72 hpf). (B) In situ hybridization with dorsal (left) and lateral (right) views of mcu expression in 3 dpf zebrafish larvae, demonstrating strong expression in the brain. (C) Quantification of mcu mRNA at 3 dpf, demonstrating similar expression levels in wt and pink1−/−. (D) RT-PCR analysis of mcu knockdown using antisense morpholino (MO) at 3 dpf, demonstrating the marked effect of MO mediated mcu k/d on mcu wild type transcript levels. (E) Q-PCR based confirmation of marked effect of MO-mediated mcu k/d on mcu mRNA transcript levels (****P < 0.0001). |
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| mcu | WT | standard conditions | 4-cell | whole organism | RTPCR | |
| Prim-5 | whole organism | RTPCR | ||||
| Long-pec | whole organism | RTPCR | ||||
| Protruding-mouth | brain | ISH | ||||
| Protruding-mouth | whole organism | RTPCR | ||||
| WT + MO3-mcu | standard conditions | Protruding-mouth | whole organism | RTPCR | ||
| pink1sh397/sh397 | standard conditions | Protruding-mouth | whole organism | RTPCR |
| Fish | Conditions | Stage | Phenotype |
|---|---|---|---|
| WT + MO3-mcu | standard conditions | Protruding-mouth | whole organism mcu expression decreased amount, abnormal |
| Acknowledgments: | ZFIN wishes to thank the journal The European journal of neuroscience for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Eur. J. Neurosci. |
|---|