ZFIN Image: Soman et al., 2017, Fig. 2

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Figure Caption

Fig. 2

Knockdown of mcu leads to rescue of dopaminergic neurons. (A) Dopaminergic (DA) neuronal cell count in wt, wt MCU (wt microinjected with MO against mcu), pink1^−/−, pink1^−/− MCU (pink1^−/− microinjected with MO against mcu) zebrafish larvae at 3 dpf. DA neuronal cell count is reduced in pink1^−/− (*P = 0.012) but completely rescued after MCU inactivation (**P = 0.0085). The scale on the y axis reflects % of DA neurons compared to wt controls. (B) Representative image showing dopaminergic neurons in the diencephalon [using tyrosine hydroxylase (TH) whole mount in situ (WISH) staining] in wt controls and pink1^−/− zebrafish injected with and without mcu MO. (C) Dopaminergic (DA) neuronal cell count in wt, wt embryos treated with Ruthenium red (RR) (wt RR), pink1^−/− and pink1^−/− embryos treated with RR (pink1^−/− RR) with complete rescue of DA neurons in pink1^−/− larvae after RR treatment (****P < 0.0001). The scale on the y axis reflects % of DA neurons compared to wt controls. (D) WT vs pink1^−/− (***P = 0.0007). Mitochondrial complex I activity is restored in 5 dpf pink1^−/− following mcu k/d (**P = 0.0071). Complex I activity is measured in μmol oxidized NADH per 1 unit of citrate synthase activity and set to 100% for the complex I activity in WT controls. The scale on the y axis reflects % of complex I activity compared to wt controls.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Neurosci.