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ZFIN ID: ZDB-FIG-170808-24
Chen et al., 2017 - Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish. Neuron   95(1):153-168.e6 Full text @ Neuron
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Fig. 7

Expression Patterns of prokr1 and prokr2 in Larval Zebrafish

(A and B) prokr1 is expressed in a row of cells in the spinal cord at 24 hpf (A, enlarged in inset) and in a bilateral cluster of cells in the ventral hypothalamus at 5 dpf (arrow in A′). Most of the embryo in (A) is overstained in order to see the weak prokr1-expressing cells in the spinal cord. prokr2 is expressed in discrete clusters of cells in the hindbrain (B), midbrain (B′ and B″), and forebrain (B′ and B″) at 5 dpf. Arrows indicate domains of prokr2-expressing cells that are shown at higher magnification in the indicated panels. Side (A), ventral (A′, B′, and B″), and dorsal (B) views are shown, with rostral at top. (B′) and (B″) show dorsal and ventral optical sections, respectively.

(C–H) prokr2-expressing neurons express vesicular glutamate transporter 2b (vglut2b) in the thalamic region (C) and glutamate decarboxylase (gad65 and gad67 probes combined) in the forebrain (E). prokr2-expressing neurons in the locus coeruleus (D) and medulla oblongata (F) express vmat:EGFP, which labels noradrenergic neurons. prokr2 is expressed in a subset of hypothalamic neurons that express hdc (G) and galn (H). Images show 0.46- (C and E), 10- (D and F), 2.3- (G), and 2.9- (H) μm-thick confocal projections. Ventral views are shown in (C)–(H), (C′)–(H′), and (C″)–(H″). (D′′′), (F′′′), (G′′′), and (H′′′) show orthogonal views of (D″), (F″), (G″), and (H″) at positions indicated by red lines. Arrows in (D) and (F)–(H) indicate representative neurons. In (C)–(H), samples were fixed at 5 dpf and are oriented with rostral at top. Scale bars in (A) and (B), 100 μm, and in (C)–(H), 10 μm. See also Figures S6 and S7.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pku2Et standard conditions Day 5 locus coeruleus norepinephrine secreting cell ISH
Day 5 medulla oblongata norepinephrine secreting cell ISH
gad1b WT standard conditions Day 5 telencephalon GABAergic neuron ISH
gad2 WT standard conditions Day 5 telencephalon GABAergic neuron ISH
galn WT standard conditions Day 5 hypothalamus anterior region ISH
hdc WT standard conditions Day 5 hypothalamus ISH
prokr1a WT standard conditions Prim-5 spinal cord anatomical region ISH
Day 5 hypothalamus ventral region ISH
prokr1b WT standard conditions Day 5 hypothalamus ISH
Day 5 hypothalamus anterior region ISH
Day 5 locus coeruleus ISH
Day 5 medulla oblongata ISH
Day 5 telencephalon ISH
Day 5 telencephalon GABAergic neuron ISH
Day 5 thalamus ISH
Day 5 thalamus glutamatergic neuron ISH
pku2Et standard conditions Day 5 locus coeruleus norepinephrine secreting cell ISH
Day 5 medulla oblongata norepinephrine secreting cell ISH
slc17a6a WT standard conditions Day 5 thalamus glutamatergic neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron