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ZFIN ID: ZDB-FIG-170807-9
Tao et al., 2017 - The pre-rRNA processing factor DEF is rate limiting for the pathogenesis of MYCN-driven neuroblastoma. Oncogene   36(27):3852-3867 Full text @ Oncogene
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 1

Loss of function of def affects sympathetic neuronal development in zebrafish. (aj) Whole-mount in situ hybridization for HuC (a and b), sox10 (c and d), phox2b (e and f), th (g and h) and dβh (i and j) in def/ mutant and sibling fish at the indicated genotypes and time points. (km) SCG signal intensity indicated by th (g and h), dβh (i and j) or EGFP (nq) in fish of different def backgrounds was calculated. Horizontal bars indicate the means±s.d., which were compared with the two-tailed unpaired t-test. ***P<0.001; ****P<0.0001. (n–q) Schematic (n) and representative images (oq) of SCG (arrows) development at 5 dpf in TgEGFP transgenic line with wild-type def (TgEGFP), def+/ (def+/;TgEGFP) or def/ (def/; TgEGFP) background. DA, diencephalic dopaminergic neurons; dpf, days post fertilization; LC, locus coeruleus; ns, not significant; R, retina; SCG, superior cervical ganglion.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh AB standard conditions Protruding-mouth superior cervical ganglion ISH
utp25hi429Tg/hi429Tg(AB) standard conditions Protruding-mouth superior cervical ganglion ISH
EGFP utp25hi429Tg/+; zdf15Tg(AB) standard conditions Day 5 superior cervical ganglion IFL
utp25hi429Tg/hi429Tg; zdf15Tg(AB) standard conditions Day 5 superior cervical ganglion IFL
zdf15Tg(AB) standard conditions Day 5 superior cervical ganglion IFL
elavl3 AB standard conditions Prim-5 whole organism ISH
utp25hi429Tg/hi429Tg(AB) standard conditions Prim-5 whole organism ISH
phox2bb AB standard conditions Prim-5 whole organism ISH
utp25hi429Tg/hi429Tg(AB) standard conditions Prim-5 whole organism ISH
sox10 AB standard conditions Prim-5 whole organism ISH
utp25hi429Tg/hi429Tg(AB) standard conditions Prim-5 whole organism ISH
th AB standard conditions Protruding-mouth superior cervical ganglion ISH
utp25hi429Tg/hi429Tg(AB) standard conditions Protruding-mouth superior cervical ganglion ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
utp25hi429Tg/hi429Tg(AB) standard conditions Prim-5 neural crest cell development process quality, normal
Protruding-mouth superior cervical ganglion dbh expression decreased distribution, abnormal
Protruding-mouth superior cervical ganglion th expression decreased distribution, abnormal
Protruding-mouth superior cervical ganglion decreased size, abnormal
Protruding-mouth superior cervical ganglion hypoplastic, abnormal
Protruding-mouth sympathetic nervous system development disrupted, abnormal
Day 5 superior cervical ganglion decreased size, abnormal
Day 5 superior cervical ganglion hypoplastic, abnormal
Day 5 sympathetic nervous system development disrupted, abnormal
utp25hi429Tg/hi429Tg; zdf15Tg(AB) standard conditions Day 5 superior cervical ganglion EGFP expression decreased amount, abnormal
Day 5 superior cervical ganglion EGFP expression decreased distribution, abnormal
Day 5 superior cervical ganglion decreased size, abnormal
Day 5 superior cervical ganglion hypoplastic, abnormal
Day 5 sympathetic nervous system development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Oncogene for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Oncogene