FIGURE

Fig. 5

ID

ZDB-FIG-170706-3

Publication

Colombo et al., 2015 - A zebrafish model of Poikiloderma with Neutropenia recapitulates the human syndrome hallmarks and traces back neutropenia to the myeloid progenitor

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Fig. 5

Effect of usb1 knockdown on haematopoiesis.

(a) Real-time PCR analysis of pu.1, gata1 and mpx expression at the indicated developmental stages of embryos injected with SMO-A or Std-MO (each at 0.6 pmol/embryo). Samples were run in triplicate and data are expressed as the mean ± standard deviation. Asterisks (*) indicate statistically significant differences (t- test; p < 0.05). (b) WISH of gata1 and mpx in SMO-A and control embryos at the indicated developmental stages. (c) O-dianisidine staining for haemoglobin in embryos injected with Std-MO (top), SMO-A (central) and SMO-B (bottom panels) (each at 0.6 pmol/embryo). The left and the right images are representative of the slight and severe erythropoiesis defects observed at the indicated percentages in SMO-A- and SMO-B-injected embryos. The # points to the caudal region of the morphant where the accumulation of blood is observed. Scale bars: (b,c) 200 μm.

Expression Data

Genes:	gata1a mpx
Fish:	AB AB + MO3-usb1
Knockdown Reagent:	MO3-usb1
Anatomical Terms:	erythroid lineage cell neutrophil whole organism
Stage Range:	26+ somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-usb1 AB + MO4-usb1
Knockdown Reagents:	MO3-usb1 MO4-usb1
Observed In:	neutrophil nucleate erythrocyte whole organism
Stage Range:	26+ somites to Long-pec

Phenotype Detail

Acknowledgments

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