ZFIN ID: ZDB-FIG-170706-4
Colombo et al., 2015 - A zebrafish model of Poikiloderma with Neutropenia recapitulates the human syndrome hallmarks and traces back neutropenia to the myeloid progenitor. Scientific Reports   5:15814 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 6

Phenotypes of morphants co-injected with SMO-A and SMO-B at subphenotypic dosage and rescue of morpholino-induced phenotypes with human USB1 RNA.

(a) Representative pictures of zebrafish embryos injected with Std-MO (0.6 pmol/embryo), SMO-A (0.3 pmol/embryo), SMO-B (0.3 pmol/embryo) and co-injected with SMO-A and SMO-B (each at 0.3 pmol/embryo). (b) Alcian blue staining at 5 dpf highlights the regular morphologic architecture of the pharyngeal arch cartilages in embryos injected with Std-MO, SMO-A and SMO-B at sub-phenotypic dosages and the aberrant cartilaginous structures in embryos co-injected with SMO-A and SMO-B (each at 0.3 pmol/embryo). (c) In the left panels, lateral views of 2 dpf embryos injected with Std-MO, SMO-A (0.6 pmol/embryo) and SMO-A and human USB1 RNA (300 pg/embryo). In the right panels, fluorescent images of tg(mpx:GFP;lyzC:dsRED) embryos at 2 dpf. The green signals, representing mpx-expressing cells, are reduced in embryos injected with SMO-A (0.6 pmol/embryo) as compared to the controls, but are enhanced in embryos co-injected with human USB1 RNA (300 pg/embryo). The fractions of embryos exhibiting the investigated phenotypes out of the total number of those examined are indicated. All images are at the same magnification. Scale bars: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP i114Tg; nz50Tg control Long-pec neutrophil IFL
i114Tg; nz50Tg + MO3-usb1 control Long-pec neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-usb1 + MO4-usb1 control Long-pec head decreased size, abnormal
Long-pec pericardium edematous, abnormal
Day 5 pharyngeal arch cartilage morphology, abnormal
i114Tg; nz50Tg + MO3-usb1 control Long-pec neutrophil GFP expression decreased amount, abnormal
Long-pec neutrophil decreased amount, abnormal
Long-pec neutrophil GFP expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.