FIGURE

Fig. 2

ID

ZDB-FIG-170706-1

Publication

Colombo et al., 2015 - A zebrafish model of Poikiloderma with Neutropenia recapitulates the human syndrome hallmarks and traces back neutropenia to the myeloid progenitor

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Fig. 2

Expression profiling of usb1 in zebrafish.

(a) Temporal and spatial usb1 expression during embryogenesis and among adult tissues. Total RNA was extracted from embryos at the indicated time-points and from the specified adult tissues, and subjected to RT-PCR with primers specific for usb1 (upper panel) or β-actin (lower panel). The sizes of the obtained PCR fragments are indicated. (b) WISH analysis of usb1. The signal obtained with usb1 antisense probe was ubiquitous until the 5-somite stage. It showed a higher intensity in the cephalic region at the 15 somites stage and displayed decreasing expression from the head to the caudal region (20 somites and 26 hpf). At 36 hpf, the signal only marked the head and the pharyngeal arches-derived structures. hpf: hour post fertilization; dpf: day post fertilization. Images showing the results obtained with sense probe are provided at each developmental stage. Scale bars: 200 μm.

Expression Data

Gene:	usb1
Fish:	AB
Anatomical Terms:	brain eye gill heart liver muscle ovary pectoral fin pharyngeal arch testis whole organism whole organism anterior region whole organism posterior region
Stage Range:	8-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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