FIGURE

Fig. 6

ID

ZDB-FIG-170706-21

Publication

Pei et al., 2016 - Additive reductions in zebrafish PRPS1 activity result in a spectrum of deficiencies modeling several human PRPS1-associated diseases

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Fig. 6

prps1a:prps1b double mutants had a reduced number of leukocytes.

(A–C) Double mutant fish had a reduced number of neutrophils. The genetically-related wild-type (A) and double mutant (B) embryos at 2 dpf were probed for mpx (neurtrophil) mRNA expression. Representative lateral view images of embryos are shown. Qualification of the data is shown in (C). prps1ab^−/− indicates prps1a:prps1b double mutant. (D–F) Double mutants have a reduced number of macrophages. Wild-type (D) and double mutant (E) embryos at 2 dpf are probed for mpeg mRNA expression (macrophages). Representative images are shown. Quantification data are shown in (F). For the quantification, leukocytes in the caudal hematopoietic tissue are counted. Graphs show the average numbers of neutrophils or macrophages per embryo. Error bars show the s.e.m. The double mutants show a significant reduction of both neutrophils and macrophage (n = 10, p < 0.001 for both).

Expression Data

Genes:	mpeg1.1 mpx
Fish:	WT prps1b^hg19/hg19; prps1a^{la015591Tg/la015591Tg}
Anatomical Terms:	macrophage neutrophil
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prps1b^hg19/hg19; prps1a^{la015591Tg/la015591Tg}
Observed In:	macrophage neutrophil
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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