FIGURE

Fig. 6

ID

ZDB-FIG-170209-42

Publication

Noack Watt et al., 2016 - The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome

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Fig. 6

Increased Tp53-dependent cell death within the neuroepithelium of polr1c^-/- and polr1d^-/- embryos.

(A-C) TUNEL staining (green fluorescence) revealed increased cell death in the cranial region and dorsal tissues of 24 hpf polr1c^-/- and polr1d^-/- mutant embryos. (D-F) Transverse sections revealed that cell death (green fluorescence) occurred primarily within the neuroepithelium. (G) qPCR (H) and Western blot analyses demonstrated the levels of tp53 and Tp53 were increased respectively in 36 hpf and 5 dpf polr1c^-/- and polr1d^-/- mutant embryos compared to controls. α-tubulin was used as a loading control. * = p < 0.05 and error bars represent 95% confidence intervals.

Expression Data

Gene:	tp53
Fish:	WT polr1c^{hi1124Tg/hi1124Tg} polr1d^{hi2393Tg/hi2393Tg}
Anatomical Term:	whole organism
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	polr1c^{hi1124Tg/hi1124Tg} polr1d^{hi2393Tg/hi2393Tg}
Observed In:	cranium apoptotic process neuroepithelial cell whole organism
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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