FIGURE

Fig. 7

ID

ZDB-FIG-170203-8

Publication

Ling et al., 2017 - Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification

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Fig. 7

Lower jaw gene expression pattern of chondrocyte and bone markers inwls,wnt9a,wnt5bandgpc4mutants. Whole-mount RNA in situ hybridization of chondrocyte differentiation markers; sox9a (A-E) and chondrocyte matrix; col2a1 (F-J). Hypertrophic chondrocyte marker; col10a1a (K-O), Bone and tendon marker; col1a2 (P-T), osteoblastic progenitor marker; runx2a (U-Y) and joint marker; bapx1 (Z-AD). (K′-L′) 40X image focused on ceratohyal (ch; left) and both cleithrum and ceratobranchial 5 (cl, cb; right). (P′-T′) 40X images focused on dentary (de; left) and ceratohyal (right). Apparent increase in sox9a, runx2a expression pattern in wls -/- with concomitant loss of col10a1a. Black arrowhead points to the position of Meckel's cartilage. Scale=50 µm..

Expression Data

Genes:	col1a2 col2a1a col10a1a nkx3-2 runx2a sox9a
Fish:	WT gpc4^{hi1688Tg/hi1688Tg} wls^c186/c186 wnt5b^{hi2735bTg/hi2735bTg} wnt9a^zf754/zf754
Anatomical Terms:	ceratobranchial 5 cartilage ceratohyal cartilage cleithrum dentary Meckel's cartilage
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wls^c186/c186
Observed In:	Meckel's cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 421(2), Ling, I.T., Rochard, L., Liao, E.C., Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification, 219-232, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.