Fig. 7

Inhibition of TGF-β signaling partially rescues M cartilage and edn1 expression in wdr68^{hi3812/hi3812} zebrafish.

(A-D) Ventral views of 5dpf Alcian stained craniofacial cartilages of zebrafish raised at 32°C and treated with DMSO or 10μM SB431542 at 14- to 15-somites stage. A) Wildtype zebrafish treated with DMSO control. Red arrow indicates M-PQ joint region. B) wdr68^{hi3812/hi3812} mutants treated with DMSO control show a lack of M cartilage. C) Wildtype zebrafish treated with 10μM SB431542 show normal craniofacial cartilage formation. D) wdr68^{hi3812/hi3812} mutants treated with 10μM SB431542 show a partial rescue of M. E) SB431542-treated mutants show a significantly reduced fraction of severe defects compared to the control group (p<0.012). (F-I) Dorsal views of edn1 ISH analysis on 22-somites stage embryos treated with DMSO or 10μM SB431542 starting at the 14- to 15-somites stage. F) Wildtype embryos treated with DMSO control. G) wdr68^{hi3812/hi3812} mutants treated with DMSO control show lack of edn1 expression. H) Wildtype embryos treated with SB431542 show similar expression compared to wild type. I) wdr68^{hi3812/hi3812} mutants treated with SB431542 show partial restoration of edn1 expression.