Fig. 1

Wdr68 is required for craniofacial development between the 17 somites and prim-5 stages.

A) Ventral view of 5dpf alcian blue stained cartilages in wild type zebrafish. M: Meckel’s PQ: Palatoquadrate. B) Mild mutant phenotype resulting from rearing of embryos at 24°C, characterized by joint fusions (arrowhead) between M and PQ. C) Severe mutant phenotype resulting from rearing of embryos at 32°C, characterized by the loss of M and PQ. D) Immunohistochemistry readily detects maternal Wdr68 protein up to the 17 somites stage in wdr68^{hi3812/hi3812} mutants raised at the permissive 24°C temperature (blue line). Maternal Wdr68 is lost by the 17 somites stage in wdr68^{hi3812/hi3812} mutants raised at the non-permissive 32°C temperature (red line). E) wdr68-MO injected non-transgenic (Non-Tg) animals display jaw defects regardless of heat shock. wdr68-MO injected Tg(hsp70l:GFP-Wdr68)^csu9 animals that are heat shocked by the prim-5 stage show rescue from jaw defects. Error bars indicate standard deviation. Additional abbreviations: Shield (Sh), 15 somites (15s), 17 somites (17s), 20 somites (20s), 25 somites (25s), prim-5 (p5), prim-25 (p25), no heat shock (-HS).