ZFIN Image: Alvarado et al., 2016, Fig. 1

Image

Figure Caption

Fig. 1

Wdr68 is required for craniofacial development between the 17 somites and prim-5 stages.

A) Ventral view of 5dpf alcian blue stained cartilages in wild type zebrafish. M: Meckel’s PQ: Palatoquadrate. B) Mild mutant phenotype resulting from rearing of embryos at 24°C, characterized by joint fusions (arrowhead) between M and PQ. C) Severe mutant phenotype resulting from rearing of embryos at 32°C, characterized by the loss of M and PQ. D) Immunohistochemistry readily detects maternal Wdr68 protein up to the 17 somites stage in wdr68^{hi3812/hi3812} mutants raised at the permissive 24°C temperature (blue line). Maternal Wdr68 is lost by the 17 somites stage in wdr68^{hi3812/hi3812} mutants raised at the non-permissive 32°C temperature (red line). E) wdr68-MO injected non-transgenic (Non-Tg) animals display jaw defects regardless of heat shock. wdr68-MO injected Tg(hsp70l:GFP-Wdr68)^csu9 animals that are heat shocked by the prim-5 stage show rescue from jaw defects. Error bars indicate standard deviation. Additional abbreviations: Shield (Sh), 15 somites (15s), 17 somites (17s), 20 somites (20s), 25 somites (25s), prim-5 (p5), prim-25 (p25), no heat shock (-HS).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One