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ZFIN ID: ZDB-FIG-161017-4
Hosseini et al., 2016 - Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science   129(18):3385-95 Full text @ J. Cell Sci.
Anatomical Terms:
Stage: Protruding-mouth

Fig. 4

Ultrastructure of correlated macrophages and neutrophils in 3D serial block-face SEM images. (A) High-resolution CLSM image of an infected tail fin after live-cell imaging and fixation. (B) SBFSEM image of the same region as in A showing correlated macrophages (arrowheads) and neutrophils (arrows). (C) Higher magnification SBFSEM images of the two macrophages and two neutrophils indicated in A and B. (D) Frames from time-lapse imaging showing the macrophages and neutrophils in C at previous time points. The macrophage (M1) with a fragmented nucleus contains a high Mm content, indicated by the asterisk in C. This macrophage phagocytized the bacterial content ~30min before fixation. Macrophage M2 contains a large aggregate of Mm which was phagocytized Mm ~1.5h before fixation. Neutrophil N1 shows a rapid signal disappearance ~45min before the fixation (D). Neutrophil N2 was recruited to the bacterial aggregate ~30min before fixation (D). Scale bars: 10µm (A-C); 2µm (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth neutrophil IFL
EGFP gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.