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ZFIN ID: ZDB-FIG-161017-4
Hosseini et al., 2016 - Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science   129(18):3385-95 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 4

Ultrastructure of correlated macrophages and neutrophils in 3D serial block-face SEM images. (A) High-resolution CLSM image of an infected tail fin after live-cell imaging and fixation. (B) SBFSEM image of the same region as in A showing correlated macrophages (arrowheads) and neutrophils (arrows). (C) Higher magnification SBFSEM images of the two macrophages and two neutrophils indicated in A and B. (D) Frames from time-lapse imaging showing the macrophages and neutrophils in C at previous time points. The macrophage (M1) with a fragmented nucleus contains a high Mm content, indicated by the asterisk in C. This macrophage phagocytized the bacterial content ~30min before fixation. Macrophage M2 contains a large aggregate of Mm which was phagocytized Mm ~1.5h before fixation. Neutrophil N1 shows a rapid signal disappearance ~45min before the fixation (D). Neutrophil N2 was recruited to the bacterial aggregate ~30min before fixation (D). Scale bars: 10µm (A-C); 2µm (D).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth neutrophil IFL
EGFP gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.