ZFIN ID: ZDB-FIG-161017-1
Hosseini et al., 2016 - Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science   129(18):3385-95 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 1

Mm infection in the zebrafish tail fin. (A) Schematic image showing the location in the tail fin where Mm was injected into zebrafish larvae at 3dpf. (B) Representative CLSM images of the infection site in the tail fin at different time points. (C) Quantification of the bacterial burden at different time points after Mm infection. (D) Numbers of macrophages (green) and neutrophils (blue) recruited to the site of infection and their infected fractions (red) per larva. (E) Normalized bacterial burden and numbers of recruited leukocytes (relative to 1hpi). The analysis shows that based on the bacterial burden, the course of infection can be divided into three different phases: the lag, exponential and stationary phase. Error bars indicate s.e.m. (n~20 larvae per time point); means with the same letter do not differ significantly (Dunnett′s post-test, P<0.05). Scale bar: 50┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth neutrophil IFL
Day 5 neutrophil IFL
EGFP gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth macrophage IFL
Day 5 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.