ZFIN ID: ZDB-PUB-160730-10
Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis
Hosseini, R., Lamers, G.E., Soltani, H.M., Meijer, A.H., Spaink, H.P., Schaaf, M.J.
Date: 2016
Source: Journal of Cell Science   129(18): 3385-95 (Journal)
Registered Authors: Meijer, Annemarie H., Schaaf, Marcel J. M., Spaink, Herman P.
Keywords: Zebrafish,Tail fin, Infection, Mycobacterium, Microscopy, Macrophage, Neutrophil, Leukocyte dynamics, Extrusion, Efferocytosis, Cell death
MeSH Terms:
  • Animals
  • Cell Death
  • Cell Movement
  • Humans
  • Imaging, Three-Dimensional
  • Larva/microbiology
  • Leukocytes/microbiology*
  • Leukocytes/pathology*
  • Macrophages/microbiology
  • Macrophages/ultrastructure
  • Models, Biological
  • Mycobacterium/physiology*
  • Mycobacterium/ultrastructure
  • Mycobacterium Infections, Nontuberculous/microbiology
  • Mycobacterium Infections, Nontuberculous/pathology
  • Neutrophils/ultrastructure
  • Phagocytosis*
  • Zebrafish/microbiology*
PubMed: 27469488 Full text @ J. Cell Sci.
Macrophages and neutrophils are the first responders to invading pathogens and contribute strongly to the host defense against intracellular pathogens. The collective interplay and dynamic interactions between these leukocytes are to a large extent uncomprehended. In the present study we have investigated their role using a combination of confocal laser-scanning and electron microscopy in a zebrafish model for tuberculosis, a local Mycobacterium marinum infection in the tissue of the larval tail fin. Our results show that neutrophils are efficient in phagocytosis of mycobacteria and that they contribute largely to their dissemination. Macrophages appear to play a major role in efferocytosis, phagocytosis of dead cells that contain bacterial content. Phagocytic cells with large bacterial aggregates are formed that can be extruded out of the tissue after cell death. Alternatively, these excessively infected cells may undergo necrosis leading to immediate recruitment of surrounding leukocytes and subsequent phagocytosis of released bacteria. Our data show that these necrotic burst events result in progression, whereas extrusion abates the infection.