ZFIN ID: ZDB-FIG-161017-3
Hosseini et al., 2016 - Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science   129(18):3385-95 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 3

Macrophages containing aggregates of Mm undergo burst or extrusion. (A) Representative images from live-cell imaging of a large aggregate of Mm (arrowhead) inside a macrophage undergoing cell death at t=3h. Spreading of Mm was observed at t=5h. The aggregate was compacted again by newly recruited leukocytes at t=8h (see Movie 6). (B) Number of burst events observed at different time points after Mm infection in the tail fin. (C) Number of extrusion events observed at different time points after Mm infection in the tail fin. (D) Representative images from live-cell imaging showing an extrusion event for accumulated Mm in a macrophage (arrowheads). First, the macrophage undergoes cell death and subsequently after 3h the bacterial content is extruded. (E) TEM image of an extruding epithelial cell from the tail fin surrounding a dead cell containing a large aggregate of Mm. The two epithelial layers and actinotrichia are indicated. (F) Higher magnification of the region indicated in E, showing compact aggregates of Mm (asterisks) in the dead cell with a condensed nucleus (arrowhead). Error bars indicate s.e.m. (n~20 larvae per time point), means with the same letter do not differ significantly (Dunnett′s post-test, P<0.05). Scale bars: 10µm (A,D,E); 2µm (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth neutrophil IFL
EGFP gl22Tg ; nz50Tg bacterial treatment by injection: Mycobacterium marinum Protruding-mouth macrophage IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.