ZFIN ID: ZDB-FIG-160824-18
Huang et al., 2016 - The circadian clock regulates autophagy directly through the nuclear hormone receptor Nr1d1/Rev-erbα and indirectly via Cebpb/(C/ebpβ) in zebrafish. Autophagy   12(8):1292-309 Full text @ Autophagy
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 1

Autophagy rhythms in zebrafish. (A) Transmission electron micrograph of the zebrafish liver sections at ZT 0, ZT 6, ZT 12 and ZT 18. The scale bar in the bottom left corner of ZT 0 panel represents one micron. High magnification in the right low corners highlights a cytosolic region. Note the presence of double-membrane autophagosomes and autolysosomes (red arrowhead). (B) Quantification of autophagosome and autolysosome abundance in (A). Data represent mean ± s.e. **, P ≤ 0.01. The Student t test was conducted. (C) RT-PCR analysis of expression of map1lc3b, bnip3, atp6v1d, becn1, ulk1a and ulk1b in zebrafish larvae from 72 hpf to 96 hpf. Zebrafish were maintained at 14 h light and 10 h dark condition. Approximately 50 zebrafish larvae were pooled for each time point. (D) RT-PCR analysis of expression of map1lc3b, bnip3, atp6v1d, becn1, ulk1a and ulk1b in zebrafish livers. Livers from 5 adult zebrafish were pooled for each time point. The mRNA expression levels were analyzed by the JTK-CYCLE method. ADJ.P for adjusted minimal P values (*, P ≤ 0 .05; ***, P ≤ 0 .001), AMP for amplitude. One-way ANOVA was conducted (***, P ≤ 0 .001). Data represent mean ± s.d. of the 3 independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp6v1d AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
becn1 AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
bnip3 AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
map1lc3b AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
ulk1a AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
ulk1b AB control Protruding-mouth to Day 4 whole organism RTPCR
Adult liver RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Autophagy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Autophagy