FIGURE

Fig. 5

ID

ZDB-FIG-160601-9

Publication

David et al., 2016 - G-quadruplexes as novel cis-elements controlling transcription during embryonic development

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Fig. 5

Effect of col2a1 G-quadruplex disruption by col2a1-ASO injection in zebrafish embryos. (A) Numbers and percentages of embryos showing dead, deformed, normal or ventral curvature phenotypes are shown in a table and represented in a stacked bar graph for 24-hpf staged embryos injected with CTRL or col2a1-ASO. ***P < 0.001, chi-square test. (B) Representative picture of 4-dpf staged larvae injected with CTRL or col2a1-ASO and stained with Alcian Blue to determine the body length. Lateral views, anterior to the left. (C) Box-plot of the relative body length of 4-dpf staged larvae injected with CTRL or col2a1-ASO. *P < 0.05, t-Student test. (D) WISH assessing the expression of col2a1 mRNA in 90% epiboly (i and ii) and 10-somite (iii and iv) staged embryos injected with CTRL (i and iii) or col2a1-ASO (ii and iv). Arrowheads point regions of lower expression. In lateral views anterior is to the left, and in dorsal and fronto-dorsal views anterior is up. Numbers and percentages of embryos/larvae with the shown phenotype are indicated in each panel. n: notochord. Scale bars (200 µm) are represented in B, Di and Diii.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.