|
Fig. 5
Effect of col2a1 G-quadruplex disruption by col2a1-ASO injection in zebrafish embryos. (A) Numbers and percentages of embryos showing dead, deformed, normal or ventral curvature phenotypes are shown in a table and represented in a stacked bar graph for 24-hpf staged embryos injected with CTRL or col2a1-ASO. ***P < 0.001, chi-square test. (B) Representative picture of 4-dpf staged larvae injected with CTRL or col2a1-ASO and stained with Alcian Blue to determine the body length. Lateral views, anterior to the left. (C) Box-plot of the relative body length of 4-dpf staged larvae injected with CTRL or col2a1-ASO. *P < 0.05, t-Student test. (D) WISH assessing the expression of col2a1 mRNA in 90% epiboly (i and ii) and 10-somite (iii and iv) staged embryos injected with CTRL (i and iii) or col2a1-ASO (ii and iv). Arrowheads point regions of lower expression. In lateral views anterior is to the left, and in dorsal and fronto-dorsal views anterior is up. Numbers and percentages of embryos/larvae with the shown phenotype are indicated in each panel. n: notochord. Scale bars (200 µm) are represented in B, Di and Diii.