FIGURE

Fig. 4

ID

ZDB-FIG-160316-10

Publication

Bühler et al., 2016 - Atrogin-1 Deficiency Leads to Myopathy and Heart Failure in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Impaired heart function in Atrogin-1-deficient embryos. (A) Fractional shortening (FS) measurements of the ventricular chambers of Atrogin-1 morphants and embryos injected with control Morpholino at 48 and 72 hpf; (B) Heart beat per minute of MO1-control compared to MO1-atrogin-1 injected embryos measured at 48 and 72 hpf. Data represent means ± SD, student´s t-test, *** p< 0.0001 (A,B); (C-F) Whole-mount antisense RNA in situ hybridization against atrial myosin heavy chain (amhc) and ventricular myosin heavy chain (vmhc) demonstrates unaltered mRNA expression in Atrogin-1 morphants at 48 hpf, scale bar = 50 µm; (G,H) H&E-stained sagittal histological sections shows normal heart morphology in MO1-control and MO1-atrogin-1 morphant hearts at 72 hpf. Atrium (A), ventricle (V), myocardium (Myo), atrio-ventricular canal (AVC) and outflow tract (OFT), scale bar = 50 µm.

Expression Data

Genes:	myh6 myh7
Fish:	WT WT + MO3-fbxo32
Knockdown Reagent:	MO3-fbxo32
Anatomical Terms:	atrium cardiac ventricle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-fbxo32
Knockdown Reagent:	MO3-fbxo32
Observed In:	atrioventricular canal atrium cardiac ventricle heart heart contraction
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.