FIGURE

Fig. S1

ID

ZDB-FIG-160316-12

Publication

Bühler et al., 2016 - Atrogin-1 Deficiency Leads to Myopathy and Heart Failure in Zebrafish

Other Figures

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Fig. S1

(A) RT-PCR for atrogin-1 and β-actin on wild-type on zebrafish cDNA from 12-Somite stage (S) until 72 hpf; (B) Western blot analysis using an anti-Myc antibody after atrogin-1 mRNA and MO2-atrogin-1 + atrogin-1 mRNA injection; (C) Immunoblot analysis using an anti-Atrogin-1 antibody after MO1-control and MO2-atrogin-1 injection; (D,E) Lateral views of control injected (D) and MO1-atrogin-1 injected embryos (E) showing birefringence at 72 hpf; (F-I) Electron micrographs of skeletal muscle cells (skm) of Atrogin-1 morphants (G,I) and embryos injected with control Morpholino (F,H) at 72 hpf. (J,J′) Immunofluorescent staining against Atrogin-1 on paraffin sections, in lateral view of wild-type embryo at 72 hpf, nuclei are counterstained with DAPI.

Expression Data

Gene:	fbxo32
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	10-13 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-fbxo32
Knockdown Reagent:	MO3-fbxo32
Observed In:	skeletal muscle
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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