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General Information
ZIRC
ZFIN ID: ZDB-FIG-151215-12
Li et al., 2015 - Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development. Journal of Biomedical Science   22:104 Full text @ J. Biomed. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 3

Nr2f1b modulates vein cell number and marker expression. a-c At 30 hpf, loss of nr2f1b function in Tg(fli1a:negfp) y7 embryos results in reduced vein cell number (b, blue bracket) as compared to uninjected wild type controls (a). The artery is marked by a red bracket. c Quantitative analysis shows a significant reduction in vein cell number in nr2f1b morphants. Compared to wild type controls. e, g, i, k, expression of the venous markers flt4 (f) and mrc1 (g) is reduced in the trunk of nr2f1b morphants at 24 hpf while there is no obvious change in the expression of arterial markers notch3 (i) and ephrinb2 (k). e′-l′ are cross sections of embryos in (e-l). d Quantification by qPCR shows a 60 % reduction in flt4 expression but no change in ephrinb2 expression in nr2f1b morphants (***refers to p < 0.0001 and **refers to p < 0.001 by an unpaired student′s t-test. Scale bars represent 50 µm in a, b, e-l and scare bar in cross-sections e′-l′ is 30 µm). Abbreviations: posterior cardinal vein (pcv), dorsal aorta (da), neural tube (nt) and pronephric ducts (pd).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
efnb2a TL control Prim-5 artery ISH
Prim-5 whole organism RTPCR
TL + MO4-nr2f1b control Prim-5 artery ISH
Prim-5 whole organism RTPCR
flt4 TL control Prim-5 vein ISH
Prim-5 whole organism RTPCR
TL + MO4-nr2f1b control Prim-5 vein ISH
Prim-5 whole organism RTPCR
mrc1a TL control Prim-5 vein ISH
TL + MO4-nr2f1b control Prim-5 vein ISH
notch3 TL control Prim-5 artery ISH
TL + MO4-nr2f1b control Prim-5 artery ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO4-nr2f1b control Prim-5 vein flt4 expression decreased distribution, abnormal
Prim-5 vein mrc1a expression decreased distribution, abnormal
Prim-5 whole organism flt4 expression decreased amount, abnormal
y7Tg + MO4-nr2f1b standard conditions Prim-15 posterior cardinal vein endothelial cell decreased amount, abnormal
Prim-15 vein cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Biomedical Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Biomed. Sci.