FIGURE

Fig. 5

ID

ZDB-FIG-151215-13

Publication

Li et al., 2015 - Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development

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Fig. 5

Nr2f1b is required for the growth of intersegmental vessels. The number of cells forming each ISV were counted in wild type control Tg(fli1a:negfp) ^y7 (a) and nr2f1b morphant embryos (b) at 30 hpf. c Proportional distribution of ISVs containing 1-7 cells in both conditions. d-j Time-lapse imaging of wild type Tg(kdrl:eGFP) ^la116 (d-f) and nr2f1b morphant (g-i) embryos to examine the extension of ISV tip cell filopodia. Confocal images at 23 hpf (red; d, g) and 26hpf (green; e, h) were merged (f, i). The extension of tip cell was quantitated by pixel intensity and shows reduced extension of tip cell in nr2f1bMO (j). k-p Time-lapse imaging of wild type Tg(fli1a:nEGFP) ^y7 (k-m) and nr2f1b morphant (n-p). Scale bars represent 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO4-nr2f1b y7Tg + MO4-nr2f1b
Knockdown Reagent:	MO4-nr2f1b
Observed In:	angioblast cell migration intersegmental vessel intersegmental vessel cell
Stage Range:	26+ somites to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.