FIGURE

Fig. 4

ID

ZDB-FIG-151030-5

Publication

Antonio et al., 2015 - The wound inflammatory response exacerbates growth of pre-neoplastic cells and progression to cancer

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Fig. 4

PGE₂ is one component of the trophic signal driving woundinflammationtriggered preneoplastic cell proliferation

A–B′ Images of Ras⁺ larval flanks from control fish bathed in Danieau′s solution or with 10 µM NS398 to block Cox2 enzyme activity, and left unwounded (A, B) or laserwounded (A′, B′) at 3 dpf before subsequent fixation at 5 dpf.

C. Graph showing quantification of preneoplastic cell numbers at 5 dpf from (A–B′) (n = 15–20 larvae in each group).

D, D′ Unwounded control Ras⁺ larvae (D) versus sibling larvae that have been laserwounded at 2 dpf (D′).

E–F′ Unwounded versus wounded larvae after injection with PU1 and GCSF morpholinos (E and E′), and addition of 20 µM dmPGE₂ (F and F′).

G. Graph showing preneoplastic cell numbers from (D–F′).

Expression Data

Gene:	EGFP
Fish:	hzm1Et; io006Tg hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r
Conditions:	chemical treatment: prostaglandin E2 chemical treatment: NS-398 resection: caudal fin chemical treatment: NS-398, laceration: trunk chemical treatment: prostaglandin E2, laceration: trunk
Knockdown Reagents:	MO1-spi1b MO3-csf3r
Anatomical Term:	trunk neoplasm
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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