FIGURE

Fig. 3

ID

ZDB-FIG-151022-22

Publication

Li et al., 2015 - Overlapping Requirements for Tet2 and Tet3 in Normal Development and Hematopoietic Stem Cell Emergence

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Fig. 3

tet2 and tet3 Have Overlapping Functions in HSC Development

(A and B) WISH for rag1 at 5 dpf. Arrow indicates thymic T cells in wild-type larvae.

(C and D) GFP labeled macrophages and neutrophils in 6 dpf larvae carrying the Tg(lysC:GFP) transgene.

(E and F) WISH for the HSC marker runx1 in the DA at 36 hpf.

(G and H) WISH for the HSC marker c-myb in the DA at 36 hpf.

(I) Number of sibling and tet2/3^DM embryos with wild-type (+++), reduced (++), or nearly absent (+) runx1 labeling in the DA. Numbers are representative of three independent crosses.

(J–M) WISH for runx1 in the DA at 32 hpf.

(N) Graph indicating the percent of tet2/3^DM larvae exhibiting high runx1 staining in the DA in uninjected controls or following injection with 100 pg mRNA encoding TET2, TET2 H1382Y.D1384A (TET2 mutant), or TET3. Numerical data are presented as the mean ± SEM.

Numbers in the lower right corner of images indicate the fraction of larvae with WISH labeling similar to the representative image. All scale bars indicate 100 µM. See also Figure S2.

Expression Data

Genes:	EGFP myb rag1 runx1
Fish:	WT tet2^mk17/mk17 tet3^mk18/mk18 nz117Tg tet2^mk17/mk17; tet3^mk18/mk18 tet2^mk17/mk17; tet3^mk18/mk18; nz117Tg
Anatomical Terms:	dorsal aorta hematopoietic stem cell macrophage neutrophil thymus T cell
Stage Range:	Prim-15 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tet2^mk17/mk17; tet3^mk18/mk18 tet2^mk17/mk17; tet3^mk18/mk18; nz117Tg
Observed In:	dorsal aorta hematopoietic stem cell hematopoietic stem cell differentiation macrophage neutrophil thymus T cell
Stage Range:	Prim-15 to Day 6

Phenotype Detail

Acknowledgments

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