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Fig. S1

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ZDB-FIG-151019-21

Publication

Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

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Fig. S1

Generation and phenotypic characterization of Tg(ACTA1-eGFP) stable lines. a) Crossing scheme to generate Tg(ACTA1-eGFP) fish. b) Confocal images show that upon iCre-mediated deletion of the loxP cassette, containing mCherry, eGFP is localized to the muscle (green) and does not overlap with other tissues (red; arrowheads). c) Maximum projection confocal microscopy images showing expression of ACTA1-eGFP in zebrafish skeletal muscle at 24, 48, and 72 hpf. Tg(ACTA1^D286G-eGFP)_high expression results in the formation of nemaline bodies (arrows) at 48 hpf and aggregates at the myosepta (arrowheads). No nemaline bodies are observed in Tg(ACTA1^wildtypeeGFP)_high skeletal muscle at any stage however globular aggregates are evident from 48 hpf at the myosepta (arrowheads).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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