ZFIN Image: Sztal et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1 Generation and phenotypic characterization of Tg(ACTA1-eGFP) stable lines. a) Crossing scheme to generate Tg(ACTA1-eGFP) fish. b) Confocal images show that upon iCre-mediated deletion of the loxP cassette, containing mCherry, eGFP is localized to the muscle (green) and does not overlap with other tissues (red; arrowheads). c) Maximum projection confocal microscopy images showing expression of ACTA1-eGFP in zebrafish skeletal muscle at 24, 48, and 72 hpf. Tg(ACTA1^D286G-eGFP)_high expression results in the formation of nemaline bodies (arrows) at 48 hpf and aggregates at the myosepta (arrowheads). No nemaline bodies are observed in Tg(ACTA1^wildtypeeGFP)_high skeletal muscle at any stage however globular aggregates are evident from 48 hpf at the myosepta (arrowheads).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Acta Neuropathol.