FIGURE

Fig. 2

ID

ZDB-FIG-151019-11

Publication

Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function

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Fig. 2

Characterization of skeletal muscle pathology in Tg(ACTA1^D286G-eGFP)_high zebrafish. a Skeletal muscle expressing i mosaic ACTA1^D286G-eGFP, and ii overlaid with a light microscopy image of the same section showing Gomori trichrome staining, and iii enlarged. Dark regions (indicative of nemaline bodies) of disrupted muscle correspond to eGFP expression (arrows). b Correlative light and electron microscopy of Tg(ACTA1^D286G-eGFP)_high fish muscle at 2 dpf. b i Fluorescent image and corresponding ii electron microscopy image of skeletal muscle section containing a dense, elongated nemaline body (arrow) and enlarged in (iii). Sections are matched using nuclei positions (asterisk, plus and hash). c i Accumulations of actin filaments (arrowheads) and ii diffuse regions of filamentous actin (arrowheads), as well as ii disrupted sarcomeric regions are evident in Tg(ACTA1^D286G-eGFP)_high skeletal muscle, at 2 dpf unlike the iii uniform sarcomeres observed in Tg(ACTA1-eGFP) zebrafish

Expression Data

Gene:	EGFP
Fish:	mnu3Tg; mnu5Tg
Anatomical Terms:	skeletal muscle skeletal muscle cellular anatomical structure
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mnu3Tg; mnu5Tg
Observed In:	skeletal muscle skeletal muscle actin filament skeletal muscle cellular anatomical structure skeletal muscle filamentous actin skeletal muscle sarcomere
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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