header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-151009-47
Li-Villarreal et al., 2015 - Dachsous1b cadherin regulates actin and microtubule cytoskeleton during early zebrafish embryogenesis. Development (Cambridge, England)   142(15):2704-18 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 4-cell to Bud

Fig. 1

Spatiotemporal expression and mutations in zebrafish dchs genes leading to pleiotropic defects during embryogenesis. (A) qRT-PCR analysis of the expression of all three zebrafish dchs genes at maternal and zygotic stages normalized to gapdh transcripts. (B) Whole-mount in situ hybridization (WISH) of dchs1b in WT embryos at four-cell, shield, 90% epiboly and 24-hpf stages. (C) Sanger sequencing trace for dchs2 A-to-T mutations. (D) Schematic of Dchs protein, with mutations denoted by asterisks. (E) qRT-PCR analysis of dchs1b expression in MZdchs1b relative to WT embryos at maternal and zygotic stages. (F) Bright-field images of WT, MZdchs1b, MZdchs2stl1/stl1 and MZdchs1bfh275/fh275;MZdchs2stl1/stl1 time-matched embryos at 1, 6 and 10hpf. Red boxes indicate distortions in the yolk cell. Large arrowheads denote yolk masses in the blastoderm. Small red arrowheads indicate anterior (A); small blue arrowheads indicate posterior (P). (G) ntl WISH for stage-matched WT, MZdchs1b and MZdchs2stl1/stl1 embryos at 70% epiboly. Inset depicts time-matched MZdchs1b embryo with a gap in the ntl expression domain. (H) Quantification of axial mesoderm length in WT (n=111), MZdchs1b (n=414) and MZdchs2stl1/stl1 (n=486) embryos. (I) hgg1 and dlx3 WISH analysis of WT, MZdchs1b and MZdchs2stl1/stl1 stage-matched embryos at two-somite stage (12hpf). (J) Quantification of the mediolateral width of dlx3 domain for WT (n=6), MZdchs1b (n=27) and MZdchs2stl1/stl1 (n=18), shown by black line.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb AB standard conditions 1-4 somites prechordal plate ISH
dchs1bfh275/fh275 standard conditions 1-4 somites prechordal plate ISH
dchs2stl1/stl1 standard conditions 1-4 somites prechordal plate ISH
dchs1a AB standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
dchs1b AB standard conditions 1-cell whole organism RTPCR
4-cell blastomere ISH
4-cell whole organism ISH
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism ISH
Shield whole organism RTPCR
90%-epiboly whole organism ISH
10-13 somites whole organism RTPCR
Prim-5 whole organism ISH
Prim-5 whole organism RTPCR
dchs2 AB standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
Sphere whole organism RTPCR
Shield whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
dlx3b AB standard conditions 1-4 somites neuroectoderm ISH
dchs1bfh275/fh275 standard conditions 1-4 somites neuroectoderm ISH
dchs2stl1/stl1 standard conditions 1-4 somites neuroectoderm ISH
tbxta AB standard conditions Shield to 75%-epiboly axial chorda mesoderm ISH
dchs1bfh275/fh275 standard conditions Shield to 75%-epiboly axial chorda mesoderm ISH
dchs2stl1/stl1 standard conditions Shield to 75%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dchs1bfh275/fh275 standard conditions 4-cell blastodisc decreased size, abnormal
4-cell blastomere size, abnormal
4-cell embryonic cleavage variability, abnormal
4-cell yolk deformed, abnormal
4-cell yolk displaced to blastodisc, abnormal
Shield embryo development delayed, abnormal
Shield yolk displaced to blastoderm, abnormal
Shield - 75%-epiboly convergent extension process quality, abnormal
Shield - 75%-epiboly notochord anterior-posterior axis decreased length, abnormal
Shield - 75%-epiboly notochord medial-lateral axis increased width, abnormal
Bud embryo development delayed, abnormal
dchs1bfh275/fh275; dchs2stl1/stl1 standard conditions 4-cell blastodisc decreased size, abnormal
4-cell blastomere size, abnormal
4-cell embryonic cleavage variability, abnormal
4-cell yolk deformed, abnormal
4-cell yolk displaced to blastodisc, abnormal
Shield embryo development delayed, abnormal
Shield yolk deformed, abnormal
Shield yolk displaced to blastoderm, abnormal
Bud embryo development delayed, abnormal
dchs2stl1/stl1 standard conditions Shield - 75%-epiboly convergent extension process quality, abnormal
Shield - 75%-epiboly notochord anterior-posterior axis decreased length, abnormal
Shield - 75%-epiboly notochord medial-lateral axis increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development