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Fig. 3
Glucocorticoid Receptor-Mediated Signaling Helps Regulate Infection-Responsive irg1 Expression within Macrophage-Lineage Cells through Induction of cebpβ Expression
(A and B) Expression of irg1 within infected DMSO (control)- and RU-486-treated (250 µM) larvae, respectively, at 1 hpi.
(C) Quantification of irg1+ cells within individual infected larvae treated with DMSO (control), 125 µM RU-486, or 250 µM RU-486 (mean ± SD).
(D and E) Expression of irg1 within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.
(F) Quantification of irg1+ cells within individual larvae as shown in (D) and (E) (mean ± SD).
(G and H) Expression of cebpβ within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.
(I) Immunofluorescence detection of Nr3c1 (Alexa 546) and Mpeg1/EGFP (Alexa 488) within DAPI-stained infected macrophage-lineage marking Tg(mpeg1:EGFP) larvae at 1 hpi. Dashed line marks Nr3c1+ nucleus.
(J) Expression of irg1 and cebpβ within dexamethasone-, hydrocortisone-, and prednisolone-injected larvae at 2 hpi.
(K) Quantification of irg1+ cells within individual larvae as shown in (J) (mean ± SD).
(L) Schematic illustrating proposed regulation of infection-responsive irg1 expression within macrophage-lineage cells. Asterisks, arrows, and arrowheads mark blood-specific, liver-specific, and gut-specific cebpβ expression, respectively. All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (A). Abbreviations: p < 0.001; p < 0.0001; n.s., not significant. See also Figure S3.
Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.