Fig. 1

The gsp locus encodes for molecular motor Myosin Vb.

Representative images of 48hpf wild-type (A,C) and gsp mutant larvae (B,D). ZO1 immuno-localisation indicates that as compared to wild type (E) cell shapes are irregular and peridermal cells are smaller in the gsp mutant (F). SEM images of wild-type (G) and gsp mutant (H) confirm the rounding-up phenotype of peridermal cells. Sequence chromatograms of gsp^NS042, gsp^AT021 and gsp^NG061 alleles (I). The asterisks in ‘I’ indicate the base substitutions in the mutant alleles. In situ hybridisation using antisense (J) and sense (K) probes against myoVb. High magnification image (L) reveal that myoVb is expressed in the head peridermal cells. A schematic (M) of domain structure of Myosin Vb indicating the positions of mutations in the three alleles. Note that in NG061 allele the splice site mutation is at 451^st aa but the truncation would occur at 475^th aa due to a frame-shift. Scale bars in G, H corresponds to 15 µ.