FIGURE

Fig. 3

ID

ZDB-FIG-150514-8

Publication

Sonal et al., 2014 - Myosin Vb Mediated Plasma Membrane Homeostasis Regulates Peridermal Cell Size and Maintains Tissue Homeostasis in the Zebrafish Epidermis

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Fig. 3

Endocytosis from apical and basolateral domain contributes to endosome and lysosome formation.

Uptake of Alexa 546 conjugated Dextran (A,B) and pHrodo Dextran (C,D) by peridermal cells in control (A,C) and myoVb (B,D) morpholino injected embryos obtained from Tg(cldnB:lynEGFP) line. Alexa 546 Dextran and pHrodo Dextran accumulates in lynEGFP vesicles in the morphants. Caveolin staining in lynEGFP expressing peridermal cells of control (E) and myoVb morphants (F) reveal that endocytosis from basolateral domain contributes for vesicle formation in morphants. X-Y plane and orthogonal projections of Alexa 546 Dextran, lynEGFP (G) and caveolin, lynEGFP (H) labelled morphant peridermal cells. Note the apical localisation of Dextran vesicles (arrowhead in G) and basolateral localization of caveolin vesicle (arrowhead in H). Scale bars are equivalent to 10 µ.

Expression Data

Antibody:	Ab1-cav1
Fish:	zf106Tg zf106Tg + MO1-myo5b
Knockdown Reagent:	MO1-myo5b
Anatomical Term:	peridermal cell vesicle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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