Fig. 5

Two-way compensatory mechanism in zebrafish embryonic epidermis.

Estimation of peridermal cell density and proliferation index in periderm and basal epidermis using BrdU labelling (A). Note the increase in number of peridermal cells per unit area, which is consistent with increase in the proliferation in the peridermal and basal epidermal cells beyond 24 and 36hpf, respectively. Lgl2 and BrdU labelling of the peridermal cells at 48hpf in wild type (B), myoVb morphant (C), p63 morphant (D) and p63,myoVb double morphant (E). Quantification (F) of cell densities and proliferation indices by BrdU labelling in periderm and basal epidermis under genetic conditions represented in B-to-E. The lynEGFP staining revealed that as compared to wild type (G) the cells are smaller in myoVb (H) and larger in p63 (I) morphants and comparable in p63,myoVb double morphants (J). Quantification of total surface area of a peridermal cell (K) at 40 hpf (for p63), 48 hpf (for PD 168393) and 50hpf (for HU) and percent survival (L) at 48hpf (for p63 and HU+Aphi), 58 hpf (for PD 168393), 74hpf (for HU) under various genetic conditions and treatments mentioned along the X-axis. Since control and myoVbMO conditions repeated in every treatment mentioned in (K), the data for these two was pooled to estimate the average. The square brackets indicate the comparison whereas asterisk indicates that the differences are statistically significant (students t test, p<0.05). The error bars represent the standard error of the mean. Scale bars in E and J correspond to 20 µ in B-E and G-J, respectively.