Fig. 2
- ID
- ZDB-FIG-150205-2
- Publication
- van der Vaart et al., 2014 - The DNA Damage-Regulated Autophagy Modulator DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense
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dram1 Is Induced by Mycobacterial Infection in a Myd88-Dependent Fashion (A) myd88+/+ and myd88-/- embryos infected with Mm were snap frozen individually at 4 dpi, and triplicate samples were compared with PBS-injected controls using a common reference microarray design. Observed differences were confirmed by RNA sequencing of pools (n = 20 embryos) of uninfected and Mm-infected myd88+/+ and myd88-/- embryos. (B) Expression of dram1 at multiple time points after infection was analyzed by qPCR on pools of Mm-infected myd88+/+ and myd88-/- embryos, relative to PBS-injected controls (mean ± SEM of n = 2 biological replicates with 20 embryos per pool). (C) Expression levels of dram1 at 4 dpi in individual myd88+/+ and myd88-/- embryos with or without infection were determined by qPCR (mean ± SEM of n = 3 biological replicates with 10 embryos per pool). (D) Macrophages, neutrophils, and leukocytes from 5-6 dpf larvae were isolated by FACS. Expression of dram1 in the positive fractions (e.g., Mpeg1+) relative to the negative fractions (-VE) was determined by qPCR (mean ± SEM of n = 4 biological replicates). See also Figure S2. |
| Genes: | (all 7) |
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| Stage Range: | Prim-5 to Day 6 |