FIGURE

Fig. 2

ID

ZDB-FIG-150205-2

Publication

van der Vaart et al., 2014 - The DNA Damage-Regulated Autophagy Modulator DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

dram1 Is Induced by Mycobacterial Infection in a Myd88-Dependent Fashion

(A) myd88^+/+ and myd88^-/- embryos infected with Mm were snap frozen individually at 4 dpi, and triplicate samples were compared with PBS-injected controls using a common reference microarray design. Observed differences were confirmed by RNA sequencing of pools (n = 20 embryos) of uninfected and Mm-infected myd88^+/+ and myd88^-/- embryos.

(B) Expression of dram1 at multiple time points after infection was analyzed by qPCR on pools of Mm-infected myd88^+/+ and myd88^-/- embryos, relative to PBS-injected controls (mean � SEM of n = 2 biological replicates with 20 embryos per pool).

(C) Expression levels of dram1 at 4 dpi in individual myd88^+/+ and myd88^-/- embryos with or without infection were determined by qPCR (mean � SEM of n = 3 biological replicates with 10 embryos per pool).

(D) Macrophages, neutrophils, and leukocytes from 5-6 dpf larvae were isolated by FACS. Expression of dram1 in the positive fractions (e.g., Mpeg1⁺) relative to the negative fractions (-VE) was determined by qPCR (mean � SEM of n = 4 biological replicates). See also Figure S2.

Expression Data

Genes:	atg5 dram1 il1b mmp9 sqstm1 tnfa tp53
Fish:	AB/TL myd88^{hu3568/hu3568}
Condition:	bacterial treatment: Mycobacterium marinum
Anatomical Terms:	lymphocyte macrophage neutrophil whole organism
Stage Range:	Prim-5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Host Microbe