FIGURE

Fig. 2

ID

ZDB-FIG-150205-2

Publication

van der Vaart et al., 2014 - The DNA Damage-Regulated Autophagy Modulator DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

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Fig. 2

dram1 Is Induced by Mycobacterial Infection in a Myd88-Dependent Fashion

(A) myd88^+/+ and myd88^-/- embryos infected with Mm were snap frozen individually at 4 dpi, and triplicate samples were compared with PBS-injected controls using a common reference microarray design. Observed differences were confirmed by RNA sequencing of pools (n = 20 embryos) of uninfected and Mm-infected myd88^+/+ and myd88^-/- embryos.

(B) Expression of dram1 at multiple time points after infection was analyzed by qPCR on pools of Mm-infected myd88^+/+ and myd88^-/- embryos, relative to PBS-injected controls (mean ± SEM of n = 2 biological replicates with 20 embryos per pool).

(C) Expression levels of dram1 at 4 dpi in individual myd88^+/+ and myd88^-/- embryos with or without infection were determined by qPCR (mean ± SEM of n = 3 biological replicates with 10 embryos per pool).

(D) Macrophages, neutrophils, and leukocytes from 5-6 dpf larvae were isolated by FACS. Expression of dram1 in the positive fractions (e.g., Mpeg1⁺) relative to the negative fractions (-VE) was determined by qPCR (mean ± SEM of n = 4 biological replicates). See also Figure S2.

Expression Data

Genes:	atg5 dram1 il1b mmp9 sqstm1 tnfa tp53
Fish:	AB/TL myd88^{hu3568/hu3568}
Condition:	bacterial treatment: Mycobacterium marinum
Anatomical Terms:	lymphocyte macrophage neutrophil whole organism
Stage Range:	Prim-5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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