FIGURE

Fig. 5

ID

ZDB-FIG-150107-17

Publication

Dworkin et al., 2014 - Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Pharyngeal and jaw defects in grhl3-morphants are rescued by edn1 mRNA. (A-F) edn1, dlx3 and hand2 are lost in grhl3-morphants but are restored (arrows) following rescue by edn1 mRNA at 24 hpf. (G-J) Lower jaw cartilage defects in grhl3-morphants are rescued by re-expression of edn1. (G and H, lateral views; I-J, ventral views; 96 hpf; ep, ethmoid plate; hs, hyosymplectic; mc, Meckel’s cartilage; pq, palatoquadrate; bh, basihyal; ch, ceratohyal; cb1-3, ceratobranchials 1-3. (K) Quantitation of rescue of lower jaw phenotypes following edn1 mRNA injection into grhl3-morphants. The proportion of phenotypically-WT fish is significantly increased in edn1-rescued grhl3-morphants; (+) compared to non-rescued morphants (-); similarly, the proportion of fish displaying either mild or severe defects is significantly reduced in edn1-rescued morphants.

Expression Data

Genes:	dlx3b edn1 hand2
Fish:	WT + MO2-grhl3
Knockdown Reagent:	MO2-grhl3
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 133, Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M., Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton, 77-90, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.