ZFIN ID: ZDB-FIG-150107-16
Dworkin et al., 2014 - Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton. Mechanisms of Development   133:77-90 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 26+ somites to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 4

grhl3 and endoderm-derived edn1 are epistatic in lower jaw development (A-L) sub-phenotypic injection of MO: edn1 together with MO: grhl3 leads to defective jaw development (A), loss of dlx3 expression in the pharyngeal arches (B) and hypoplastic facial cartilage at 96 hpf (C, lateral view; D, ventral view). These defects are not observed in sub-phenotypic co-injection of either MO: edn1 (E and H) or MO: grhl3 (I-L) with MO: control. (M-O) The expression of edn1 at 22 hpf in grhl3-morphants (M) is similar to that in endoderm-deficient sox32-morphants (N); both morphants continue to express edn1 in the ectoderm of the first arch (green arrows) but exhibit greatly reduced or absent edn1 expression in the endodermal pouches of both the first and subsequent arches (red arrows) relative to controls (O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT + MO1-edn1 standard conditions Day 4 otic vesicle ISH
Day 4 peripheral olfactory organ ISH
Day 4 pharyngeal arch ISH
WT + MO1-edn1 + MO2-grhl3 standard conditions Day 4 otic vesicle ISH
Day 4 peripheral olfactory organ ISH
Day 4 Not Detected pharyngeal arch ISH
WT + MO2-grhl3 standard conditions Day 4 otic vesicle ISH
Day 4 peripheral olfactory organ ISH
Day 4 pharyngeal arch ISH
edn1 WT control 26+ somites pharyngeal arch 1 ectoderm ISH
26+ somites pharyngeal pouch ISH
WT + MO2-grhl3 standard conditions 26+ somites pharyngeal arch 1 ectoderm ISH
26+ somites pharyngeal pouch ISH
WT + MO2-sox32 standard conditions 26+ somites pharyngeal arch 1 ectoderm ISH
26+ somites pharyngeal pouch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-edn1 + MO2-grhl3 standard conditions Day 4 basihyal cartilage aplastic, abnormal
Day 4 cartilaginous joint aplastic, abnormal
Day 4 ceratobranchial cartilage aplastic, abnormal
Day 4 ceratohyal cartilage hypoplastic, abnormal
Day 4 Meckel's cartilage hypoplastic, abnormal
Day 4 palatoquadrate arch hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 133, Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M., Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton, 77-90, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.