FIGURE

Fig. 3

ID

ZDB-FIG-150107-15

Publication

Dworkin et al., 2014 - Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton

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Fig. 3

Endothelin-1 is a direct target of grhl3. (A) Micro-ChIP assay from 24 hpf zebrafish expressing grhl3 (as a negative control for FLAG-Ab binding) or grhl3-FLAG shows an enrichment in binding at three out of four predicted binding sites in the edn1 promoter in the grhl3-FLAG-injected group. Sites are shown relative to the transcription start site (TSS). No enrichment in binding is seen at predicted site 2, or within the negative-control (Exon 2, coding) region. (B-G) The expression of edn1 (B, C) is lost in grhl3 morphants at 24 hpf, as is expression of dlx3 (D, E) and hand2 (F, G), although note persistent expression of dlx3 in the olfactory placode (op) and otic vesicles (ov), and hand2 within the heart (h). (1-2; pharyngeal arches 1-2).

Expression Data

Genes:	dlx3b edn1 hand2
Fish:	WT WT + MO2-grhl3
Knockdown Reagent:	MO2-grhl3
Anatomical Terms:	heart tube olfactory placode otic vesicle pharyngeal pouch
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 133, Dworkin, S., Simkin, J., Darido, C., Partridge, D.D., Georgy, S.R., Caddy, J., Wilanowski, T., Lieschke, G.J., Doggett, K., Heath, J.K., Jane, S.M., Grainyhead-like 3 regulation of endothelin-1 in the pharyngeal endoderm is critical for growth and development of the craniofacial skeleton, 77-90, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.