FIGURE

Fig. 4

ID

ZDB-FIG-141125-5

Publication

Choksi et al., 2014 - Systematic discovery of novel ciliary genes through functional genomics in the zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

FIGs are required for both ciliogenesis and cilia organization. (A-H) Cilia of the pronephric duct were stained with anti-Arl13b antibodies (green) and the basal bodies were stained with antibodies to γ-tubulin (red) in 24hpf embryos. (A) Long cilia of uniform length and orientation are visible in a wild-type embryo. (B-G) Six morphants exhibited shortening of pronephric cilia at 24hpf. (H) The knockdown of mustn1a caused curling of cilia (arrows) and a disorganization of the γ-tubulin (arrowheads). Scale bar: 10μm. (I) Measurements of ciliary length in wild-type versus morphant embryos for six genes reveals the extent of ciliary shortening in the morphants. Error bars represent s.e.m. ***P<5.0×10⁷ (Student′s t-test, two-tailed, P-values are listed in supplementary material Table S3); n≥30.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-lect2.1 AB + MO1-magi3b AB + MO1-mustn1a AB + MO1-si:ch211-71m22.1 AB + MO1-si:dkey-26i13.8 AB + MO1-si:dkey-165a24.9 AB + MO1-si:dkey-224e22.2
Knockdown Reagents:	MO1-lect2.1 MO1-magi3b MO1-mustn1a MO1-si:ch211-71m22.1 MO1-si:dkey-26i13.8 MO1-si:dkey-165a24.9 MO1-si:dkey-224e22.2
Observed In:	pronephric duct cilium pronephros ciliary basal body pronephros cilium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development