Fig. 5

FIGs are required for ciliary motility. (A,B) Embryos were injected with RNA encoding Arl13b-GFP to label KV cilia and imaged using high framerate video microscopy. (A) An overlay of 20 frames from a movie from a control embryo indicates four moving cilia in view. (B) By comparison, overlays from movies of an embryo with immotile cilia show no blurring, indicating stationary objects. Scale bar: 5μm. (C) Measurements of ciliary motility in wild-type and morphant embryos targeting ten selected genes. Two aspects of ciliary motility were scored: the percentage of KV cilia that exhibited motility (green) and the average beat frequency of motile cilia (blue). Error bars represent s.e.m. ***P<4.0×10⁷ (Fisher′s Exact test, two-tailed; P-values are listed in supplementary material Table S3). **P<5.0×10³ (Student′s t-test, two-tailed, P-values are listed in supplementary material Table S3). The three genes without reported ciliary beat frequency did not have sufficient motile cilia for analysis (n<8).