FIGURE

Fig. S11

ID

ZDB-FIG-141007-53

Publication

Bielczyk-Maczy?ska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis

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Fig. S11

To assess the HSC emergence in the aorta-gonad-mesonephros (AGM) region, we injected gene-specific MOs in single-cell stage Tg(c-myb:GFP) transgenic embryos. For kalrn1, mfn2, pdia5, psmd13 and wasplb MO injected embryos no difference in the number of HSCs was observed when compared to the control at 30 hpf. However, kalrn2 and rcor1 depleted embryos had a marked decrease in the number of HSCs at 30 hpf, implying an important role of these genes in specification of HSCs in the AGM. Representative images of the AGM region are shown. All embryos are oriented with anterior to the left and dorsal to the top.

Expression Data

Gene:	EGFP
Fish:	zf169Tg zf169Tg + MO1-kalrna zf169Tg + MO1-kalrnb zf169Tg + MO1-pdia5 zf169Tg + MO1-psmd13 zf169Tg + MO1-waslb zf169Tg + MO2-mfn2 zf169Tg + MO2-rcor1 (all 8)
Knockdown Reagents:	MO1-kalrna MO1-kalrnb MO1-pdia5 MO1-psmd13 MO1-waslb MO2-mfn2 MO2-rcor1 (all 7)
Anatomical Term:	ventral wall of dorsal aorta hematopoietic stem cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf169Tg + MO1-kalrnb zf169Tg + MO2-rcor1
Knockdown Reagents:	MO1-kalrnb MO2-rcor1
Observed In:	hematopoietic stem cell ventral wall of dorsal aorta
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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