Image
|
Figure Caption
Fig. S11
To assess the HSC emergence in the aorta-gonad-mesonephros (AGM) region, we injected gene-specific MOs in single-cell stage Tg(c-myb:GFP) transgenic embryos. For kalrn1, mfn2, pdia5, psmd13 and wasplb MO injected embryos no difference in the number of HSCs was observed when compared to the control at 30 hpf. However, kalrn2 and rcor1 depleted embryos had a marked decrease in the number of HSCs at 30 hpf, implying an important role of these genes in specification of HSCs in the AGM. Representative images of the AGM region are shown. All embryos are oriented with anterior to the left and dorsal to the top.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ PLoS Genet.