ZFIN ID: ZDB-FIG-141007-44
Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis. PLoS Genetics   10:e1004450 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 1

In vivo morpholino screen in zebrafish identifies 15 new regulators of thrombopoiesis.

MOs were injected into one-cell stage transgenic Tg(cd41:EGFP) zebrafish embryos and assayed for their effect on the number of thrombocytes (cd41high) at 3 dpf. Representative confocal images were taken of the CHT. For akap10, brd3a, brf1b, kalrn 1, kalrn 2, kif1b, mfn2, pdia5, psmd13 and satb1 a severe decrease in the number of cd41high positive cells was observed. brf1a, rcor1, waspla, wasplb and wdr66 depletion resulted in a mild phenotype, and fen1, grtp1b and tmcc2 MO injected embryos showed no phenotype. All embryos are oriented with anterior to the left and dorsal to the top. White arrow – thrombocytes; white arrowhead – HSCs.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Protruding-mouth thrombocyte IFL
la2Tg + MO1-akap10 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-brd3a standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-brf1a standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-brf1b standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-fen1 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-grtp1b standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-kalrna standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-kalrnb standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-pdia5 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-psmd13 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-satb1b standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-tmcc2 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-wasla standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-waslb standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-wdr66 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO2-mfn2 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO2-rcor1 standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO4-kif1b standard conditions Protruding-mouth thrombocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-akap10 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-brd3a standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-brf1a standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-brf1b standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-kalrna standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-kalrnb standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-pdia5 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-psmd13 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-satb1b standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-wasla standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-waslb standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-wdr66 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO2-mfn2 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO2-rcor1 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO4-kif1b standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.