ZFIN ID: ZDB-FIG-141007-46
Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis. PLoS Genetics   10:e1004450 Full text @ PLoS Genet.
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 5

brd3a is an important regulator of thrombopoiesis.

(A) Live confocal imaging of zebrafish embryos injected with hBRD3-GFP mRNA revealed the nuclear localization of hBRD3 and that it binds to mitotic chromosomes (white arrows). Expression of hBRD3 in brd3a MO injected embryos resulted in a partial rescue of the number of thrombocytes as shown in (B). (C) A graph to illustrate the number of thrombocytes in control, splice brd3a MO and splice brd3a MO plus hBRD3 mRNA injected embryos. Each dot represents the number of thrombocytes in the individual MO-injected embryos with respect to control. A blue horizontal line represents the mean value of the number of thrombocytes for each group of embryos. Student t test, * p = 0.016; n = 17. All embryos are oriented with anterior to the left and dorsal to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Protruding-mouth thrombocyte IFL
la2Tg + MO1-brd3a standard conditions Protruding-mouth thrombocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-brd3a standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
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