ZFIN ID: ZDB-FIG-141007-63
Bielczyk-Maczyńska et al., 2014 - A loss of function screen of identified genome-wide association study Loci reveals new genes controlling hematopoiesis. PLoS Genetics   10:e1004450 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. S22

To assess the role of other BET family members in thrombopoiesis MO knock down of brd2a, brd2b and brd4 was performed. A) Although morphologically normal, embryos injected with brd2a, brd2b or brd4 MOs had a severe decrease in the number of thrombocytes at 3 dpf (B). Representative pictures of CHT are shown. C) For splice-blocking MOs the effect of the MOs was confirmed by RT-PCR. All the embryos are positioned with anterior to the left and dorsal to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP la2Tg control Protruding-mouth thrombocyte IFL
la2Tg + MO1-brd2a standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-brd2b standard conditions Protruding-mouth thrombocyte IFL
la2Tg + MO1-brd4 standard conditions Protruding-mouth thrombocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2Tg + MO1-brd2a standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-brd2b standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
la2Tg + MO1-brd4 standard conditions Protruding-mouth thrombocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.