|ZFIN ID: ZDB-FIG-141007-63|
To assess the role of other BET family members in thrombopoiesis MO knock down of brd2a, brd2b and brd4 was performed. A) Although morphologically normal, embryos injected with brd2a, brd2b or brd4 MOs had a severe decrease in the number of thrombocytes at 3 dpf (B). Representative pictures of CHT are shown. C) For splice-blocking MOs the effect of the MOs was confirmed by RT-PCR. All the embryos are positioned with anterior to the left and dorsal to the top.
|la2Tg + MO1-brd2a||standard conditions||Protruding-mouth||thrombocyte||IFL|
|la2Tg + MO1-brd2b||standard conditions||Protruding-mouth||thrombocyte||IFL|
|la2Tg + MO1-brd4||standard conditions||Protruding-mouth||thrombocyte||IFL|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.|