ZFIN ID: ZDB-FIG-140919-14
Ordas et al., 2013 - MicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection. BMC Genomics   14(1):696 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Prim-15

Fig. 6

Increased infection-induction of apolipoprotein gene expression under conditions of combined miR-146a and miR-146b knockdown. (A-F) Embryos were injected with control morpholino (scMO) or with a combination of 146aMO1 and 146bMO1 (146a/bMOs) and infected with S. typhimurium as described in Figure 5. Gene expression levels of six apolipoprotein genes, apoa1a (A), apoa4 (B), apoba (C), apobb (D), apoc1l (E), and apoeb (F), were analyzed by qPCR in two replicate infection experiments (1 and 2) and relative expression levels are shown with the lowest expression level set at 1. Data are the mean ± SEM of triplicate qPCR measurements. Asterisks indicate significant differences (*, P < 0.05; **, P <0.01) determined by an un-paired t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
apoa1a AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
apoa4b.1 AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
apoba AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
apobb.1 AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
apoc1 AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
apoeb AB/TL control Prim-5 to Prim-15 whole organism RTPCR
AB/TL + MO1-mir146b + MO3-mir146a bacterial treatment: Salmonella enterica subsp. enterica serovar Typhimurium Prim-5 to Prim-15 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics